| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sommer, Truly (7)
|
MAUN |
24.40 |
24.98 |
0.58 |
| 2 |
Hamill, Emily (7)
|
MAUN |
29.94 |
25.65 |
-4.29 |
| 3 |
Kern, Abigail B (7)
|
MAGPAC |
30.26 |
26.32 |
-3.94 |
| 4 |
Hetzer, Reese O (7)
|
MAGPAC |
29.80 |
27.89 |
-1.91 |
| 5 |
DeLorenzo, Gabriela A (7)
|
MAPCAC |
|
27.93 |
- |
| 6 |
Kellner, Gabriella H (7)
|
MACBST |
|
29.82 |
- |
| 7 |
Dagostino, Storylynn A (7)
|
MAGPAC |
31.88 |
30.27 |
-1.61 |
| 8 |
Jackson, Kaelynn B (7)
|
MAGPAC |
|
33.91 |
- |
| 9 |
White, Gabrielle R (7)
|
MAPCAC |
37.76 |
37.25 |
-0.51 |
| 10 |
Replogle, Abigail (7)
|
MAUN |
|
44.41 |
- |
|
Landis, Sylvia A (7)
|
MAFSS |
|
DQ |
- |
|
Parrott, Agatha R (7)
|
MAFSS |
|
DQ |
- |
|
Kudless, Sienna M (7)
|
MAGPAC |
|
DQ |
- |
|
Schwarzwalder, Emily R (7)
|
MAGPAC |
34.41 |
NS |
- |